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Blood, Vol. 112, Issue 2, 264-276, July 15, 2008
Coactivator function of RIP140 for NF B/RelA-dependent cytokine gene expression
Blood Zschiedrich et al.
112: 264
Supplemental materials for: Zschiedrich et al
Files in this Data Supplement:
- Figure S6. Coomassie Brilliant Blue staining of GST/GST-RelA proteins precipitated in GST pulldown assays as indicated (JPG, 40.5 KB)
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Arrows mark corresponding GST-RelA variants.
- Figure S7. Coomassie Brilliant Blue staining of GST/GST-RelA proteins precipitated in GST pulldown assays as indicated (JPG, 33.2 KB)
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Arrows mark corresponding GST-RelA variants.
- Figure S8. Co-immunoprecipitation of HEK293T cells transfected with an NFkB family member expression vector plus Flag-RIP140 or an empty Flag vector using anti-FLAG M2 antibody (JPG, 77.6 KB)
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Bound proteins were resolved by SDS-Page and subsequently detected by Western blot using FLAG M2, RelA, RelB, c-Rel, and p50 antibodies.
- Figure S9. Transient transfection assay of 3T3-L1 cells co-transfected with 3xCRE Luc (containing a multimerized CREB binding site) or pκB-Luc (containing wild-type NFκB binding sites) together with RelA (pκB-Luc) and RIP140-specific or non-specific control shRNA constructs as indicated (JPG, 54.4 KB)
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Cells were stimulated for 24 h with cAMP agonist forskolin (10 µM) (3xCRE Luc) to trigger CREB/CBP-dependent transcription. Data are means ± s.e.m (n=6). *, p < 0.05.
- Figure S10. Coomassie Brilliant Blue staining of GST/GST-CBP proteins precipitated in GST pulldown assays as indicated (JPG, 31.3 KB)
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Arrows mark corresponding GST-CBP deletion variants.
- Figure S11. Coomassie Brilliant Blue staining of GST/GST-CBP proteins precipitated in GST pulldown assays as indicated (JPG, 22.5 KB)
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Arrows mark corresponding GST-CBP deletion variants.
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