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Blood, Vol. 112, Issue 3, 848-855, August 1, 2008
Resolvin E1, an EPA-derived mediator in whole blood, selectively counterregulates leukocytes and platelets
Blood Dona et al.
112: 848
Supplemental materials for: Dona et al
Files in this Data Supplement:
- Table S1. RvE1 selectively regulates specific cytokine/chemokine production in human whole blood (PDF, 89.6 KB)
- Figure S1. RvE1 did not stimulate reactive oxygen species production by PMNs or monocytes in whole blood (JPG, 63.5 KB)
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Human whole blood was incubated with the ROS indicator carboxy –H2DCF-DA for 15 min and then challenged with increasing concentrations of either fMLP (diamond) or RvE1 (square) for 15 min. The results represent the mean values ±SEM (n=3) for ROS generation by PMN (solid line) or monocytes (dotted line) in whole blood as a percentage of the maximum response obtained from the fMLP challenge (1 µM, 15 min, 37°C, pH 7.45). *, P< 0.05; **, P <0.005.
- Video 1. Intravital videomicrocopy scene of rolling leukocytes in a mouse cremaster muscle venule (MOV, 3.95 MB)
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Numerous rolling leukocytes can be seen as large bright spheres that were fluorescently tagged by i.v. injection of Rhodamine 6G. The small, fast-moving fluorescent particles are platelets, which also accumulate this fluorescent dye. The movie was taken under video-triggered stroboscopic epi-illumination through a ×40 water immersion objective (Zeiss) and recorded on a digital video camera (Rolera MGI EMCCD camera). The counter in the upper left corner shows the duration of the recording.
- Video 2. The same field of view as in Video 1 shown 10 min. after injection of RvE1 (100 ng i.v.) (MOV, 2.8 MB)
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Recording parameters were the same as during the control period.
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