|
|
Blood, Vol. 112, Issue 5, 1951-1959, September 1, 2008
Sµ mutation patterns suggest different progression pathways in follicular lymphoma: early direct or late from FL progenitor cells
Blood Ruminy et al.
112: 1951
Supplemental materials for: Ruminy et al
Files in this Data Supplement:
- Table S1. Somatic mutations at the 5′Sµ in FL samples (PDF, 189 KB) -
Patients are identified by their unique patient number (UPN), and all samples by their type (LN: lymph node, PB: peripheral blood, BM: Bone marrow, PF: peritoneal fluid, ASC: autologous stem cells) and the time from diagnosis. Mutations are presented following the nomenclature for the description of sequence variation from the human genome variation society (HGV). Lowercase letters were used when ICV showed minor clones. *: identify samples where more than one clone was identified. When deletions (del) or duplications (dup) resulted in overlapping electropherograms, the estimated percentage of different clones is given between brackets (min: minor).
- Figure S1. Genealogical trees constructed from individual subclones (JPG, 484 KB)
-
For two patients (#990 here), PCR products from LN, BM and PB were subcloned and sequenced. For these two cases, the mutations identified from whole PCR products or from individual subclones are presented in tables (a) and (b) respectively. The mutations shared by all tumoral cells are shown in red. In both cases, two main branches of evolution were identified. The mutations that discriminate these branches are shown in blue and green. Few mutations, identified only by one or the other approach are shown in black. The evolution trees constructed either from whole PCR products or from individual subclones are presented in panels (c) and (d) respectively. In panels (d), subclones are identified by their origin (LN, BM or PB) and numbers. P: Precursors not isolated among the subclones. Both approaches identified the same major branches (the different subpopulations defined in panels (c) are boxed in panels (d) (populations A, B, C, and D), confirming the clonal evolution of the tumors. These results show that clonal evolution in FL can be accurately reconstructed with a limited number of sequencing reaction performed on whole PCR products. They also indicate that this latter approach does not reflect the true clonal complexity of the disease, as only subcloning experiments allow the identification of individual cells.
- Figure S2. Genealogical trees constructed from individual subclones (JPG, 355 KB)
-
For two patients (#655 here), PCR products from LN, BM and PB were subcloned and sequenced. For these two cases, the mutations identified from whole PCR products or from individual subclones are presented in tables (a) and (b) respectively. The mutations shared by all tumoral cells are shown in red. In both cases, two main branches of evolution were identified. The mutations that discriminate these branches are shown in blue and green. Few mutations, identified only by one or the other approach are shown in black. The evolution trees constructed either from whole PCR products or from individual subclones are presented in panels (c) and (d) respectively. In panels (d), subclones are identified by their origin (LN, BM or PB) and numbers. P: Precursors not isolated among the subclones. Both approaches identified the same major branches (the different subpopulations defined in panels (c) are boxed in panels (d) (populations A, B, and G), confirming the clonal evolution of the tumors. These results show that clonal evolution in FL can be accurately reconstructed with a limited number of sequencing reaction performed on whole PCR products. They also indicate that this latter approach does not reflect the true clonal complexity of the disease, as only subcloning experiments allow the identification of individual cells.
|
|
