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Blood, Vol. 112, Issue 5, 1971-1980, September 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Gossypol, a BH3 mimetic, induces apoptosis in chronic lymphocytic leukemia cells Blood Balakrishnan et al. 112: 1971 Supplemental materials for: Balakrishnan et al Files in this Data Supplement: Table S1. Patient information (PDF, 86.7 KB) Table S2. Effect of pan-caspase inhibitor on gossypol-induced apoptosis (PDF, 17.5 KB) Figure S1. Gossypol-induced PARP cleavage is not blocked by Z-VAD (JPG, 26.4 KB) - CLL cells were treated with or without Z-VAD in both the presence and absence of the indicated concentrations of gossypol for 24 hours. PARP protein was immunoblotted as described in Patients and Methods. Figure S2. Decrease in the ATP pool after treatment with gossypol (JPG, 59.5 KB) - CLL cells were treated with 30 µM gossypol for the indicated time. Nucleotides were extracted using the perchloric acid method and analyzed by high-pressure liquid chromatography as described in Patients and Methods. Figure S3. Gossypol treatment does not displace Mcl-1 and Bcl-2 into cytosol (JPG, 39.9 KB) - CLL cells were treated with or without gossypol at the indicated concentrations for 24 hours. Mcl-1 and Bcl-2 protein expression was studied in whole cell extract (WCE) and the cytosolic fraction using immunoblot method as described in Patients and Methods. Actin and GAPDH were used as loading controls. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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