|
|
Blood, Vol. 112, Issue 7, 2917-2926, October 1, 2008
Myc regulates aggresome formation, the induction of Noxa, and apoptosis in response to the combination of bortezomib and SAHA
Blood Nawrocki et al.
112: 2917
Supplemental materials for: Nawrocki et al
Files in this Data Supplement:
- Figure S1. Knockdown of c-Myc and treatment with cycloheximide decrease protein synthesis in NCI-H929 cells (JPG, 29 KB)
-
Following transfection with siRNA or cycloheximide treatment, NCI-H929 cells were incubated with 35Scysteine/35Smethionine for 30 minutes. Translation rates were determined as described in the Materials and Methods. Mean (n = 3), SD. *Indicates a significant difference from non-target (NT) siRNA exposed untreated cells, p < 0.05.
- Figure S2. Real-time PCR analysis of HDAC6 levels in HFF cells expressing Myc-ER (JPG, 23.3 KB)
-
The cells were treated for 24 h with 4-HT and RNA was harvested for real-time PCR analysis. GAPDH was used as an internal control. Mean (n = 3), SD. *Indicates a significant difference from MSCV-IRES-puromycin cells, p < 0.05.
- Figure S3. Bortezomib induces a unique type of ER stress characterized by an absence of eif2α phosphorylation (JPG, 30.7 KB)
-
Cells were pre-treated with 1 µM 4-HT for 24 h to activate Myc-ER. Cells were subsequently treated with 100 nM bortezomib, 2 µM SAHA, or both for 24 h. Cells were collected and lysed and immunoblotting was performed to determine the levels of Bip, p-eif2α, eif2α, and actin as described in the Materials and Methods.
- Figure S4. Activation of Myc-ERTAM sensitizes HFF cells to ER stress (JPG, 29.1 KB)
-
Cells were pre-treated with 1 µM 4-HT for 24 h to activate Myc-ER. Cells were subsequently treated with 5 µg/ml tunicamycin or brefeldin A for 24 h. Drug-induced apoptosis was measured by PI-FACS analysis. Mean (n = 3), SD. *Indicates a significant difference from MSCV-IRES-puromycin cells, p < 0.05.
|
|
