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Blood, Vol. 112, Issue 10, 4028-4038, November 15, 2008
IRF8 regulates B-cell lineage specification, commitment, and differentiation
Blood Wang et al.
112: 4028
Supplemental materials for: Wang et al
Files in this Data Supplement:
- Table S1. DNA sequences for PCR assays (PDF, 43.4 KB)
- Figure S1. qPCR analysis of gene expression in MP cells sorted from IRF8+/+ and IRF8−/− mice (JPG, 17.3 KB)
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The data is representative of three experiments with similar results.
- Figure S2. The number of pre–pro-B cells in IRF8+/+ and IRF8−/− mice (JPG, 23.2 KB)
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BM cells from the indicated mice were stained for B220, CD19, CD43, DX5, and LY6C. The frequency (left panel) and the absolute number (right panel) of pre–pro-B cells (B220+CD19−CD43+DX5−LY6C−) are shown as mean ± SEM of 6 mice per group. *p<0.001 compared with the control group.
- Figure S3. qPCR analysis of gene expression in cultured myeloid-lymphoid progenitor cells (JPG, 36.7 KB)
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HSC of wild-type mice were cultured with SCF, IL-3, IL-6 and Flt3L for 18 h before they were transfected with retroviral vectors encoding IRF8-GFP, K79E-GFP, or GFP only for 24 h in the presence of SCF, IL-3, IL-6, Flt3L, IL-7 and GM-CSF. GFP+ cells were sorted and mRNA of indicated genes was measured by qPCR. Data (mean ± SD) represents two independent experiments.
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