|
|
Blood, Vol. 112, Issue 2, 383-393, July 15, 2008
Down-regulation of TCF8 is involved in the leukemogenesis of adult T-cell leukemia/lymphoma
Blood Hidaka et al.
112: 383
Supplemental materials for: Hidaka et al
Files in this Data Supplement:
- Table S6. Primers for semi-quantitative RT-PCR (PDF, 17.9 KB)
- Table S7. Primers for quantitative RT-PCR (PDF, 13.1 KB)
- Table S8. Primers for genomic sequences of TCF8 promoter after bisulfite treatment (PDF, 12 KB)
- Table S9. Primers for chromatin immunoprecipitation by anti-acetylated histone H3 or H4 antibodies of TCF8 promoter (PDF, 12.4 KB)
- Table S10. Primers for amplification of the TCF8 genomes (PDF, 35.4 KB)
- Figure S1. The expression profiles of TCF8, ITGB1 and EPC1 mRNA by DNA microarray (JPG, 46.1 KB)
-
Three samples of CD4+ or CD4+CD45RO+ T lymphocytes from normal volunteers and 8 samples of ATLL cells in the peripheral blood were used for determining the gene expression profiles. The relative expression rates of all of the genes in the Affymetrix oligonucleotide microarray were scaled by the global scaling method to adjust the target intensity to 300. Two patients (indicated by asterisks) have the chromosome 10p11.2 abnormalities.
- Figure S2. The expression profiles of the genes mapped within the deletion region at 10p11 (JPG, 48.2 KB)
-
Semi-quantitative RT-PCR was performed using various types of T-lymphoblastic leukemia cell lines. Jurkat and MOLT4 are T-lymphoid leukemia cell lines, MT2 and HUT102 are HTLV-1-infected cell lines, and ED, KOB, SO4, KK1, Su9T, and S1T are ATLL cell lines. Three ATLL cell lines (indicated by asterisks) are presented the deletion of chromosome 10p11.2 with TCF8.
- Figure S3. Recovered expression of TCF8 gene in ATLL cells treated with 5-aza-dC or hydralazine (JPG, 53.2 KB)
-
Cell lines were cultured with 10 µmol/L of 5-aza-dC for 72 hours, or with 10 µmol/L of hydralazine for 72 hours. After the treatments, total RNA was extracted from the cells and semi-quantitative RT-PCR was performed with TCF8 and β-actin.
|
|
