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Blood, Vol. 112, Issue 4, 1280-1289, August 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Endothelial CD47 interaction with SIRP is required for human T-cell transendothelial migration under shear flow conditions in vitro Blood Stefanidakis et al. 112: 1280 Supplemental materials for: Stefanidakis et al Files in this Data Supplement: Video 1. The role of endothelial CD47 in T-cell transmigration under shear flow (MOV, 756 KB) - Endothelial CD47 was blocked with B6H12 mAb in an in vitro assay as detailed in Methods. DIC microscopy images were acquired every 15 sec for 10 min as detailed in Methods. In the presence of this mAb, CD3+ T cells were rounded-up, failed to spread and to polarize on TNF-α stimulated endothelium, an event that is required for a successful leukocyte migration. The arrow indicates a single T cell that exhibits the defective migration phenotype. Video 2. Adhesion and migration of CD3+ T cells of control Class I mAb treated TNF-α activated endothelium under flow (MOV, 406 KB) - DIC microscopy images were acquired every 15 sec for 10 min as detailed in Methods. T cells identified with an asterisk (*) at the start of the movie are examples of cells that polarize, migrate on the apical surface for various lengths of time before transmigrating. The T cell identified with an arrow polarizes, migrates and eventually transmigrates. The frames in which T cells transmigration begins are marked with asterisks (*). Video 3. The role of SIRPγ in T-cell transmigration under shear flow (MOV, 1.23 MB) - T-cell SIRPγ was blocked with LSB2.20 mAb in an in vitro assay as detailed in Methods. DIC microscopy images were acquired every 15 sec for 10 min as detailed in Methods. The arrow indicates a single T cell that exhibits the defective migration phenotype. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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