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Blood, Vol. 112, Issue 3, 652-660, August 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef c-Cbl expression levels regulate the functional responses of human central and effector memory CD4 T cells Blood Brembilla et al. 112: 652 Supplemental materials for: Costantino Brembilla et al Files in this Data Supplement: Table S1. Qualitative analysis of the spot geometry (PDF, 12.7 KB) - Circularity and diameter were evaluated in a total of 249 spots by calculating the Coefficient of Variation (CV) of individual (tot.) or triplicate (tripl.) spots. 100% refers to a perfectly circular spot. Figure S1. Surface expression of TCR/CD3ε, CD28 and co-receptors in memory T cell subsets (JPG, 55.4 KB) - PBMC were stained with CCR7 and CD45RA and the expression of co-receptors, CD28 and TCR/CD3 measured by FACS at saturating concentrations of antibodies in the different CD4 (left panel) or CD8 (right panel) T cell subsets. Shown are summed data of seven subjects. Figure S2. Expression of signaling molecules in presence of CTP-c-Cbl transduction (JPG, 59.2 KB) - Following transduction with CTP-c-Cbl, purified EM cells were stimulated with -CD3 (5 µg/ml) and -CD28 (1 µg/ml) antibodies for 16 hours. Signaling components were quantified by RPP arrays and normalized to -actin. Colours indicate the mean log2 ratios of signaling components relative to resting non-transduced EM cells in 3 distinct subjects. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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