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Blood, Vol. 112, Issue 5, 1794-1803, September 1, 2008
The familial Mediterranean fever protein, pyrin, is cleaved by caspase-1 and activates NF- B through its N-terminal fragment
Blood Chae et al.
112: 1794
Supplemental materials for: Chae et al
Files in this Data Supplement:
- Figure S1. Subcellular localization of GFP-labeled N330 (JPG, 16.1 KB)
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PT67 cells were transfected with GFP-tagged N330. After 24 h, cells were fixed, counterstained with DAPI, and visualized using a fluorescent microscope N330 was found in both the nucleus and the cytoplasm, appearing as a distinctive filamentous network in the nucleus.
- Figure S2. Effect of calpain I inhibitors on N330-induced IκB-α degradation (JPG, 143 KB)
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HeLa cells were cotransfected with IκB-α-V5 and N330-myc, and treated with varying amounts of calpain inhibitors, calpastatin human domain I (0.625-5 µg/ml, calpain I inhibitor), calpeptin (3.125-12.5 µg/ml, calpain I and II inhibitor), and PD15060 (3.125-25 µg/ml, calpain I and II inhibitor). Cells were also transfected with N330-myc or IκB-α-V5 alone as controls. Cell lysates were analyzed by Western blotting with anti-V5 Ab for IκB-α (top), anti-myc Ab for N330 (middle), or anti-β-tubulin Ab for loading controls (bottom). Asterisks denote degraded ~30-kDa IκB-α fragment.
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