SEARCH:   Advanced

Blood, Vol. 113, Issue 8, 1661-1669, February 19, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Menin regulates the function of hematopoietic stem cells and lymphoid progenitors Blood Maillard et al. 113: 1661 Supplemental materials for: Maillard et al Files in this Data Supplement: Figure S1. Efficient Cre-mediated Men1 inactivation after administration of Tamoxifen to CreERTm+ Men1f/f mice (JPG, 39.3 KB) - PCR was performed on DNA isolated from total BM or purified Gr1+CD11b+ myeloid cells. Arrows point the bands corresponding to amplification of the Cre-excised allele, floxed allele or wild-type Men1 allele. Figure S2. Impaired hematopoietic recovery of menin-deficient mice after administration of 5-fluoro-uracil (5-FU) (JPG, 74.3 KB) - The bone marrow was analyzed 6 days after a single dose of 5-FU (150 mg/kg). (A) Decreased recovery of Gr1+CD11b+ myeloid cells in menin-deficient CreERTm+ Men1f/f as compared to control CreERTm− Men1f/f mice. (B) Decreased recovery of Lin−c-KithiSca-1− cells (arrows), containing committed myeloid progenitors (CMP, GMP) in the absence of menin. Figure S3. Representative examples of peripheral blood myeloid chimerism illustrating Fig. 6 (JPG, 55.2 KB) - Analysis of the contribution of CD45.2+ cells to blood myeloid Gr1+CD11b+ cells at baseline and 16 weeks after administration of TAM to mixed bone marrow chimeras (CD45.1+ competitor and control CD45.2+ CreERTm− Men1f/f bone marrow, or CD45.1+ competitor and CD45.2+ CreERTm+ Men1f/f menin-deficient bone marrow). A representative example is shown. Arrows point to a significantly decreased percentage of menin-deficient as compared to control myeloid cells. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020