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Blood, Vol. 112, Issue 5, 2017-2019, September 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Leukemic transformation in mice expressing a NUP98-HOXD13 transgene is accompanied by spontaneous mutations in Nras, Kras, and Cbl Blood Slape et al. 112: 2017 Supplemental materials for: Slape et al Files in this Data Supplement: Table S1. PCR conditions (PDF, 12.1 KB) Table S2. Regions analyzed for mutation (PDF, 11.3 KB) Figure S1. Sequence chromatogram of N-ras codon 12 (boxed) from wild type mouse 8055 (A), and GGT → GAT mutations (arrows) in two mutant mice 251 and 1892 (B and C, respectively) (JPG, 32 KB) - Figure S2. Expression of a Nras G12D mutant leads to IL3 independence of an IL3-dependent NHD13 cell line (JPG, 74 KB) - (A) RT-PCR demonstrating expression of cloned Nras cDNAs. One primer was designed to the 3′ UTR of the expression vector such that endogenous Nras would not be detected. (B) Cells expressing wild type Nras (i) or G12D mutant Nras (ii) on day 18 following withdrawal of IL-3. (C) Growth curve showing live cell density in cultures expressing wild type Nras (blue circles) or G12D mutant Nras (red squares). WT = wild type; EV = empty vector. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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