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Blood, Vol. 112, Issue 4, 1317-1324, August 15, 2008
Long-term expansion of effector/memory V 2–  T cells is a specific blood signature of CMV infection
Blood Pitard et al.
112: 1317
Supplemental materials for: Pitard et al
Files in this Data Supplement:
- Figure S1. Representative CD3, pan-δ, and Vδ2 triple staining obtained in one CMV-seronegative and one CMV-seropositive donor. Only CD3+ cells are shown (JPG, 38.6 KB)
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- Figure S2. CD107a stainings of two representative Vδ2neg γδ T cells cultured on fibroblasts infected or not with CMV (JPG, 34.5 KB)
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Shown are results obtained with two representative cell lines isolated from one CMV− and one CMV+ subject. Indicated numbers are percentages of CD107a+ cells among the γδ T cells.
- Figure S3. Freshly collected blood from CMV− or CMV+ blood donors was stained for six-color flow cytometry with mAbs directed against CD3, γδ TCR, Vδ2, CD45RA, CD27, and either CD28 or CD62L (JPG, 170 KB)
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Representative profiles of Vδ2pos and Vδ2neg γδ T cells in one CMV− and one CMV+ donor.
- Figure S4. Freshly collected blood from 11 CMV+ blood donors was stained with mAb directed against CD3, pan-δ, Vδ2, Vδ3, CD27, and CD45RA (JPG, 87.6 KB)
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Results represent (A) the percentage of Vδ1 (black bars) or Vδ3 cells (grey bars) among Vδ2neg T cells, or (B) the percentage of Vδ1 (black bars) or Vδ3 cells (grey bars) expressing a TEMRA phenotype, in each donor.
- Figure S5. Freshly collected blood from CMV− or CMV+ blood donors was stained with mAbs directed against CD3, γδ TCR, Vδ2, CD45, CD27, and either CD94, NKG2A or CD158 (anti-CD158a/h, b/j, i and e added simultaneously) (JPG, 99.5 KB)
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Intracellular staining of perforin and granzyme B were also performed after membrane marker labelling. Only phenotypes of the major populations of Vδ2neg γδ T cells are shown (i.e. naïve cells in CMV− donors and TEMRA cells in CMV+ donors).
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