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Blood, Vol. 113, Issue 3, 555-562, January 15, 2009
Both alleles of PSF1 are required for maintenance of pool size of immature hematopoietic cells and acute bone marrow regeneration
Blood Ueno et al.
113: 555
Supplemental materials for: Ueno et al
Visualization of crypt base columnar (CBC) cells
Twelve-week-old, female PSF1+/− and wt mice were injected intraperitoneally at 4 h intervals with 0.2 ml of BrdU solution (5 mg/ml) in PBS. After 16 h, the small intestine were dissected, fixed in 4% paraformaldehyde in PBS, embedded in paraffin, and sectioned. Samples were stained with anti-BrdU antibody, as described.1, 2 REFERENCES
1. Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007; 449:1003-1007.
2. Ueno M, Itoh M, Kong L, Sugihara K, Asano M, Takakura N. PSF1 is essential for early embryogenesis in mice. Mol Cell Biol. 2005;25:10528-10532.
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