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Blood, Vol. 112, Issue 10, 4193-4201, November 15, 2008
An 86-probe-set gene-expression signature predicts survival in cytogenetically normal acute myeloid leukemia
Blood Metzeler et al.
112: 4193
Supplemental materials for: Metzeler et al
Files in this Data Supplement:
- Document 1. Study participants (PDF, 25.4 KB)
- Table S1. List of the 86 Affymetrix probe sets contained in the prognostic gene signature (PDF, 84.4 KB) -
For each probe set, the univariate Cox score for the association of the expression values with overall survival in the training set is given. Probe sets with a score of >2.9 were included in the prognostic signature. Weights were assigned to each probe set by principal components analysis. To calculate the prognostic scores for patients in an independent dataset, the expression values for these 86 probe sets first are centered to a mean of 0. Next, the expression values are rescaled for each probe set individually, so that their standard deviations match the ones in the training dataset, as listed in the table. Finally, the prognostic score for each patient is calculated as the sum of the 86 rescaled expression values, each multiplied with the corresponding weight.
- Table S2. Association of baseline clinical and molecular characteristics with the continuous gene expression risk score (PDF, 621 KB)
- Figure S1. Probe set – wise comparison of mean and standard deviation of the expression values in the training and test cohorts (JPG, 54 KB)
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163 AML cases in the training cohort were analyzed on HG-U133 A&B chips, while 79 patients in the test cohort were analyzed on HG-U133 plus 2.0 microarrays. For each of the 44754 probe sets common to both microarray types, the figure shows the difference of the mean expression values between the A & B and plus 2.0 arrays, plotted against the ratio of the observed standard deviations. Samples analyzed on the HG-U 133 plus 2.0 chips tended to have lower mean expression values and higher standard deviations for most of the probe sets. Red dots identify the 86 probe sets that are contained in our prognostic signature.
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