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Blood, Vol. 113, Issue 8, 1723-1729, February 19, 2009
AKT inhibitor, GSK690693, induces growth inhibition and apoptosis in acute lymphoblastic leukemia cell lines
Blood Levy et al.
113: 1723
Supplemental materials for: Levy et al
Files in this Data Supplement:
- Figure S1. Effect of PI3K inhibitor, ZSTK474, on the growth of ALL cell lines and normal lymphocytes (JPG, 15.9 KB)
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ALL cell lines, A3, and Tanoue, along with primary human CD4+ peripheral T cells and mouse thymocytes, were treated with various concentrations of ZSTK474 for 72 h. Cell proliferation was measured, as described in materials and methods. Graphs represent a curve fit analysis of the dose response data.
- Figure S2. AKT stimulation in ALL cell lines, A3, and Tanoue (JPG, 10.8 KB)
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Cells were plated overnight in serum-free media, followed by treatment with 5 ng/ml of insulin for 30 min. Cell lysates were analyzed by western blotting for phospho-AKT and total AKT for loading control. Figure shows comparison of untreated and GSK690693-treated cells.
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