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Blood, Vol. 113, Issue 1, 175-185, January 1, 2009
cIAP1-dependent TRAF2 degradation regulates the differentiation of monocytes into macrophages and their response to CD40 ligand
Blood Dupoux et al.
113: 175
Supplemental materials for: Dupoux et al
Files in this Data Supplement:
- Figure S1. Fluorescence microscopy analysis (JPG, 191 KB)
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Fluorescence microscopy analysis of TRAF2 (Red) and cIAP1 (green) (A) or TRAF2 (Red) and GM130 (Green) (B) expression in TPA-differentiated THP-1 cells (24 hours), in the presence of 30 µM MG132 (A) or 10 µM Lactacystin (B) added for the 7 or 4 last hours of treatment respectively. When indicated, cells were incubated with irrelevant primary antibody and specific secondary antibody. The nuclei were stained using Hoechst (Blue). Original magnification ×500.
- Figure S2. Flow cytometry analysis of transduction efficacy (JPG, 62.3 KB)
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Cells were transduced either with a control vector (white areas) or with the IRES-GFP pMXs-IG vector encoding GFP alone, TRAF2* - GFP, c-IAP1ΔRING - GFP and c-IAP1 - GFP (grey areas). Percentage of transduced cells and mean fluorescence intensities (MFI) are indicated.
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