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Blood, Vol. 113, Issue 1, 149-153, January 1, 2009
AT-101 induces apoptosis in CLL B cells and overcomes stromal cell–mediated Mcl-1 induction and drug resistance
Blood Balakrishnan et al.
113: 149
Supplemental materials for: Balakrishnan et al
Files in this Data Supplement:
- Table S1. CLL patient characteristics and AT-101-induced apoptosis (PDF, 52.1 KB)
- Figure S1. Effect of AT-101 and fludarabine on Bcl-2 and Bcl-xl protein levels in CLL cells (from patient #30) growing in suspension culture (JPG, 77.2 KB)
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CLL lymphocytes were incubated with either 0, 10, 20 µM AT-101 for 4, 8, 24, 48 and 72hr (A) or with 10 µM fludarabine for 8, 24, or 72 hrs (B) and the expression of Bcl-2 and Bcl-xl was measured by immunoblotting. The annexin positivity for each sample is given above.
- Figure S2. Effect of AT-101 or fludarabine on PARP cleavage and Mcl-1 decrease in CLL cells (from patient #31) in suspension culture or on stromal co-culture (JPG, 67 KB)
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CLL lymphocytes in suspension culture or on stroma were incubated with 20 µM AT-101 for 72 hr (A) or 10 µM fludarabine for 72 hr (B) and the PARP cleavage and the Mcl-1 protein levels were analyzed by immunoblotting.
- Figure S3. Effect of fludarabine on ERK and AKT proteins in CLL cells (from patient #30) growing in suspension (JPG, 53.6 KB)
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CLL B cells were cultured in the presence or absence of 10 µM fludarabine for 8, 24, and 72 hrs and the expression of phosphor and total ERK and AKT were measured by immunoblotting.
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