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Blood, Vol. 112, Issue 13, 5193-5201, December 15, 2008
A key role for G-CSF–induced neutrophil production and trafficking during inflammatory arthritis
Blood Eyles et al.
112: 5193
Supplemental materials for: Eyles et al
Files in this Data Supplement:
- Video S1. In vivo confocal microscopy of a post-capillary venule from a cremaster muscle acutely exposed to G-CSF (MOV, 1.87 MB)
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PE-conjugated anti–Gr-1 mAb was used to stain neutrophils, and APC-conjugated anti-CD45 mAb to stain all leukocytes, such that neutrophils appear magenta. Both neutrophils (Gr-1+, CD45+; magenta) and other leukocytes (Gr-1−, CD45+ blue) can be seen undergoing rolling interactions in the venule, whereas only neutrophils are arrested on the endothelial surface. AlexaFluor 488-conjugated isolectin GS-IB4 from Griffonia simplicifolia was used to label the microvasculature, strongly staining adjacent capillaries, and adjacent muscle fibres are visible via autofluorescence.
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