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Blood, Vol. 112, Issue 7, 2722-2729, October 1, 2008
Lymphoid-affiliated genes are associated with active histone modifications in human hematopoietic stem cells
Blood Maës et al.
112: 2722
Supplemental materials for: Maes et al
Files in this Data Supplement:
- Table S1. List of primers used for real-time PCR analysis of ChIP experiments (PDF, 40.9 KB)
- Figure S1. Histone H3 and H4 acetylation values relative to B2-microglobulin or GAPDH control genes (JPG, 212 KB)
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ChIP analyses of CD34+CD38lo, GpA+ and T cell progenitors with antibodies to acetylated histone H4 (A, B), and H3 (C, D) at representative B lymphoid (B), T lymphoid (T), erythroid (E), myeloid (M), non-hematopoietic (N), β2-microglobulin (β2m) and GAPDH genes. Results are shown as enrichment values (bound/input) relative to the ubiquitous B2m (white bars) or GAPDH (black bars) genes.
- Figure S2. Histone H3 and H4 acetylation along the CD79B locus in CD34+38lo, CD19+ and GpA+ cells (JPG, 42.5 KB)
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Top: Map of CD79B gene showing positions of exons (black rectangles), DNaseI hypersensitive sites (HS) and primers used for PCR analysis. Bottom: ChIP analysis of multipotent CD34+38lo, B-committed CD19+ and erythroid GpA+ precursors with antibodies to acetylated histone H4 (solid line) and H3 (dotted line). Results are shown as enrichment values (bound/input) relative to the ubiquitous B2m gene and are means and standard deviations of 2 to 3 independent ChIP experiments analyzed in triplicate.
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