|
|
Blood, Vol. 112, Issue 10, 4184-4192, November 15, 2008
The biologic properties of leukemias arising from BCR/ABL-mediated transformation vary as a function of developmental origin and activity of the p19ARF gene
Blood Wang et al.
112: 4184
Supplemental materials for: Wang et al
Files in this Data Supplement:
- Figure S1. Additional southern blot analyses of leukemias arising in Arf null cells transduced by the p210 BCR/ABL retrovirus (JPG, 56.8 KB)
-
The 10 individual animals analyzed in this figure (one animal per lane) serve to supplement the six animals (lanes 1–6) show in Fig. 3. The methods and analyses are identical to those described for Fig. 3. The lane labeled M is molecular weight markers, from top 10, 8, 6, 5, 4, 3, 2, and 1.5 kilobases.
- Figure S2. Multi-parameter FACS analysis using precursor–B-cell markers of normal (wild type bone marrow) and leukemia (HSC-derived and Pro-Derived) cell populations (JPG, 184 KB)
-
(A) Gated B220 low CD43+ populations have been further subdivided according to relative expression of BP-1 and CD24. In WT mice and GFP negative cell populations, Fraction A (CD24low BP-1low ), Fraction B (CD24high, BP-1low) and Fraction C (CD 24high BP-1high) are readily detected. The GFP+ population in HSC-derived ALL have substantial populations of cells meeting the classical criteria for Fractions B and C. (B) Due to the heterogeneity of expression of B220, CD43, and CD24, representative fractions of B220 and CD43 are shown with respect to BP-1 and CD24 surface antigen density. There are insufficient GFP negative populations in ProB-ALL mice amenable to such analysis (data not shown).
|
|
