|
|
Blood, Vol. 112, Issue 9, 3735-3743, November 1, 2008
Neutralization of tumor-derived soluble Glucocorticoid-Induced TNFR-Related Protein ligand increases NK cell anti-tumor reactivity
Blood Baltz et al.
112: 3735
Supplemental materials for: Baltz et al
Files in this Data Supplement:
- Figure S1. Effects of sGITRL on NK cell cytotoxicity and cytokine production (JPG, 61.7 KB)
-
Cytotoxicity of NK cells was evaluated by chromium release assays with the indicated GITRL-positive or -negative tumor cells. (A) Experiments were performed in the absence or presence of the indicated concentrations of sGITRL with C1R-neo supernatant as control. (B) Cytotoxicity assays in control medium (diamonds) or with 10ng/ml sGITRL derived from C1R-GITRL supernatant in the absence (filled squares) or presence of 10ng/ml human IgG1 (circles) or GITR-Ig fusionprotein (triangles). The effect on NK cell IFN-γ production was determined by incubation of NK cells for 24h with or without the indicated GITRL-positive or GITRL-negative tumor cells and analysis of supernatants by ELISA. (C) Cultures in the absence or presence of the indicated concentrations of sGITRL with C1R-neo supernatant as control. (D) Cultures with or without 10ng/ml sGITRL derived from C1R-GITRL supernatant in the absence or presence of 10ng/ml human IgG1 or GITR-Ig fusionprotein.
- Figure S2. Direct effects of sGITRL on tumor cells (JPG, 70.8 KB)
-
Expression of GITR on the indicated tumor cells and NK cells was determined by real time PCR (A) and FACS analysis (B) as previously described.21 In addition, tumor cells were cultured for the indicated times alone, with 10ng/ml sGITRL or control supernatant. After medium exchange, chromium release assays with NK cells (E:T ratio 30:1) were performed (C).
|
|
