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Blood, Vol. 113, Issue 10, 2245-2255, March 5, 2009
Ex vivo characterization of polyclonal memory CD8+ T-cell responses to PRAME-specific peptides in patients with acute lymphoblastic leukemia and acute and chronic myeloid leukemia
Blood Rezvani et al.
113: 2245
Supplemental materials for: Rezvani et al
Files in this Data Supplement:
- Figure S1 (JPG, 79.9 KB)
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(A) ELISPOT assay after short-term expansion. Freshly thawed PBMCs from patient 21 were assayed by IFN-γ ELISPOT assay directly (top row) or after 1 week of expansion in the presence of 0.1 µM of PRAME peptide. The day 1 assay was performed using 200,000 cells/well, and the day 7 assay was performed using the expanded cells derived from 67,000 of freshly thawed PBMCs/well. Each well represents one of 5 replicate wells. The first number below each image represents the raw number of spots counted in the displayed well and the number in parentheses represents the number of peptide-specific spots/million freshly thawed PBMCs calculated using data from all 5 replicates. Neg indicates the number of spots in this set of wells did not meet criteria for significant difference from control wells. (B) The top panel illustrates the frequency of IFN-γ responding CD8+ T cells prior to expansion and the bottom panel represents the frequencies of IFN-γ responding CD8+ T cells following 3 × weekly stimulation with test peptide.
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