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Blood, Vol. 112, Issue 6, 2390-2399, September 15, 2008
Antibody fucosylation differentially impacts cytotoxicity mediated by NK and PMN effector cells
Blood Peipp et al.
112: 2390
Supplemental materials for: Peipp et al
Files in this Data Supplement:
- Table S1. Summary of glycosylation profile of investigated batches (PDF, 103 KB) -
Structures of neutral N-linked glycans detected on 2F8-C or 2F8-H. Explanation of symbols: squares, N-acetylglucosamine; closed circles, mannose; open circles, β1,4-galactose; striped circle, α1,3-galactose; diamond, N-glycolylneuraminic acid (NeuGc); triangle, α1,6-fucose. Hex, Hexose; HexNac, N-acetylhexosamine; Deoxyhex, deoxyhexose; M5, oligomannose-5 type glycan. n.d.: not determined.
- Figure S1. Analysis of the N-linked glycosylation profile of glycovariants (JPG, 53.8 KB)
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N-linked glycans of 2F8 glycovariants were enzymatically released and analysed by HPAEC-PAD. (A) 2F8-C and 2F8-H analyzed before and after β-galactosidase treatment. (B) Analyses of 2F8-HEK (F+/G−/S−) and 2F8-C (F−/G−/S−). The retention time (min) is indicated on the abscissa, the detector response (nC) is indicated on the ordinate. Peaks 2, 4, 6 and 7 represent non-core fucosylated glycans. Peaks eluting at (+) and (++) represent mono-sialylated and di-sialylated variants of neutral glycans eluting at (0).
- Figure S2. Analyses of Fc/Fc receptor interactions using recombinantly expressed extracellular domains of different Fcγ receptors and exo-glycosidase treated 2F8 batches (JPG, 104 KB)
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Binding profiles of 2F8-HEK-(F+/G−/S−) (□), 2F8-C-(F−/G−/S−) (■), mock-treated 2F8-HEK-(F+/G−/S−) (□, dashed line) and mock-treated 2F8-C-(F−/G−/S−) (■, dashed line) to various Fcγ receptors measured by ELISA. 2F8-C F(ab′)2 fragments (▼) are shown as a control. One representative experiment out of three is shown.
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