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Blood, Vol. 112, Issue 9, 3563-3573, November 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef HMG-CoA reductase inhibition induces IL-1β release through Rac1/PI3K/PKB-dependent caspase-1 activation Blood Kuijk et al. 112: 3563 Supplemental materials for: Kuijk et al Files in this Data Supplement: Figure S1. Simvastatin treatment does not affect TNF-α secretion (JPG, 32 KB) - THP-1 cells were cultured for 24 hours in the presence or absence of simvastatin (10 µM) and stimulated for an additional 4 hours with 200 ng/ml LPS. Release of TNF-α was determined by ELISA; data are represented as means ± SEM (n=3). Figure S2. Solvent DMSO does not affect IL-1β secretion (JPG, 40.5 KB) - THP-1 cells were cultured for 24 hours in the presence or absence of simvastatin (10 µM) and stimulated for an additional 4 hours with 200 ng/ml LPS. DMSO was added either one hour prior to simvastatin or one hour prior to LPS treatment. Release of IL-1β was determined by ELISA; data are represented as means ± SEM (n=4). * = p<0.01 compared to 100%. Figure S3. Serum starvation does not affect PKB phosphorylation (JPG, 41 KB) - THP-1 cells were serum-starved overnight and cultured for up to 48 hours. Cells were lysed by adding hot sample buffer. Cell lysates were assayed for phospho-PKB content. Actin content served as control for equal protein loading. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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