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Blood, Vol. 112, Issue 8, 3164-3174, October 15, 2008
Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in the contractile ring and Rho/Rock signaling
Blood Lordier et al.
112: 3164
Supplemental materials for: Lordier et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 65.8 KB)
- Figure S1. 3D imaging of the nucleus of 4N interphase MKs (JPG, 76.2 KB)
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Cells were stained for Lamin A and C (green) and Tubulin α (red). A fraction of the cells presented a single nucleus (bi-lobulated or mono-lobulated) (V-VI), another fraction two separated nuclei (I-II) and the third fraction two side by side nuclei but it remained difficult to assume that they were completely separated (III-IV).
- Figure S2. An endomitotic polyploid MK showed attenuated furrow ingression compared to dipolar endomitotic MK (A) and another one showed the absence of furrow ingression (B) (JPG, 186 KB)
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Time-lapse images were obtained by confocal video microscopy. The cell was stained with CellTracker Orange and serial images were obtained at 3 min interval.
- Figure S3. Four slices were scanned on one MK at 30 minutes after beginning of endomitosis, there were 8 daughter cells connected together (JPG, 60.5 KB)
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- Video 1. Time-lapse images corresponding to Fig. 1A (MOV, 2.27 MB)
- Video 2. Time-lapse images corresponding to Fig. 1B (MOV, 1.85 MB)
- Video 3. Time-lapse images corresponding to Fig. 7A (MOV, 428 KB)
- Video 4. Time-lapse images corresponding to Fig. 7B (MOV, 105 KB)
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