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Blood, Vol. 112, Issue 6, 2520-2528, September 15, 2008
Retention of Plasmodium falciparum ring-infected erythrocytes in the slow, open microcirculation of the human spleen
Blood Safeukui et al.
112: 2520
Supplemental materials for: Safeukui et al
Files in this Data Supplement:
- Table S1. Akaike Information Criterion (AIC), half-life values, and coefficient of variation (CV) of the parameter estimates for each isolated-perfused spleen experiment, using the different models (one- or two-compartment model, without or with a residua -
Data are from circulation experiments (A–F, Fig. 2 and Fig. S1) or circulation-recirculation experiments (e′ and f′, Fig 3 b1 & b2).
- Figure S1. Parasitaemia of P. falciparum FUP schizont-iRBCs (△ triangles), and ring- iRBCs (● circles) in the perfusate during 120 minutes of perfusion (6 independent experiments) (JPG, 69.7 KB)
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- Figure S2. Surface immunofluorescence using a pool of hyperimmune sera from adult African patients (1/100 dilution) of cultured schizont-iRBC (A1) and ring-iRBC (A2) populations prepared for experiments and counter-stained with Hoechst (JPG, 54.9 KB)
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The typical Giemsa-stained aspect is shown on inserts. Most schizont-iRBCs were intensely stained whereas no ring-iRBC was stained (bar = 10 µm). (A3) PAGE auto-radiographs of Triton-SDS extracts from surface-iodinated cultured schizont-iRBCs (S), and ring-iRBCs (R). The 290 kDa band (arrow) probably corresponding to PfEMP1, was observed only in the schizont-iRBC extract.
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