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Blood, Vol. 112, Issue 10, 4202-4212, November 15, 2008
MYC stimulates EZH2 expression by repression of its negative regulator miR-26a
Blood Sander et al.
112: 4202
Supplemental materials for: Sander et al
Files in this Data Supplement:
- Table S1. Murine MYC-induced cell lines included in the study (XLS, 17.5 KB)
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For more details, see also Marinkovic et al.18,19
- Table S2. Filtered miRNA expression data (XLS, 180 KB)
- Table S3. miR-26a response signature (list of significant gene expression changes following over-expression of miR-26a) (XLS, 186 KB)
- Figure S1. miRNA expression changes over time following MYC down-regulation (JPG, 61.9 KB)
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(A) and (B) Northern Blot analysis following doxycycline treatment at indicated time points shows down-regulation of miR-17-5p and miR-20a. Interestingly, there was rapid miRNA precursor decay with delayed down-regulation of mature miRNAs.
- Figure S2. Correlation of miRNA based clustering with commonly activated pathways (JPG, 92.2 KB)
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(A) Enlarged view of the sample dendrogram of the average linkage cluster of MYC-induced untreated lymphoma cell lines (n=26; see also Fig. 1C) with cluster-defined lymphoma subgroups highlighted as indicated (see colored bars). T-cell lines are highlighted in blue. (B) Western Blot analyses for ARF, BCL2, and (phosphorylated) AKT. Boxes underneath samples indicate the sample location in (A) based on cluster-defined grouping.
- Figure S3. Functional evaluation of the inducible miRNA expression system (JPG, 45.1 KB)
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(A) and (B) 24 hrs following Dox treatment inducible miR-26a expression in transfected Raji and Namalwa cells was confirmed by assaying GFP expression by FACS analysis, as well as quantitative RT-PCR for mature miR-26a.
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