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Blood, Vol. 113, Issue 4, 911-918, January 22, 2009
Maintenance of the BMP4-dependent stress erythropoiesis pathway in the murine spleen requires hedgehog signaling
Blood Perry et al.
113: 911
Supplemental materials for: Perry et al
Files in this Data Supplement:
- Figure S1. Expression of Desert hedgehog in the spleen during the recovery from acute anemia (JPG, 17.8 KB)
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(A) Spleens were isolated from mice 24 hours after PHZ treatment. The spleens were fixed and processed for paraffin sections. The sections were stained with anti-BMP4 (Novacastra Labs/Vector Labs, Burlingame, CA) and anti-Dhh (Santa Cruz Biotechnology, Santa Cruz, CA) antibodies as previously described. Sections were analyzed by confocal microscopy using an Olympus FV300 confocal microscope. (B) The expression of Dhh protein in the spleen during the recovery from acute anemia was done by analyzing lysates from homogenized spleens by western blot. Spleens were isolated on the indicated days and homogenized in RIPA buffer. Lysates were run on SDS-PAGE gels and western blotted. The Blots were probed with anti-Dhh antibody (Santa Cruz Biotechnology, Santa Cruz, CA). The blots were stripped and re-probed with anti-b-actin (Santa Cruz Biotechnology, Santa Cruz, CA) as a loading control.
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