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Blood, Vol. 112, Issue 4, 1231-1239, August 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses Blood Gurer et al. 112: 1231 Supplemental materials for: Gurer et al Files in this Data Supplement: Figure S1. Genetic engineering of the carboxyl-terminus of EBNA1 into the heavy chain of the αDEC-205 antibody (JPG, 66.7 KB) - (A) Schematic depiction of the fusion between the αDEC-205 antibody and the EBNA1400–641 protein domain. (B) αDEC-205 and EBNA1 conjugated to αDEC-205 or an IgG2b isotype control resolved by SDS-PAGE gel electrophoresis followed by silver staining. (C) Immunoblotting with anti-EBNA1 antibody to confirm the presence of EBNA1 in the conjugates. Molecular weights in kD are indicated on the left. Figure S2. αDEC-205-EBNA1 fusion antibody targets DCs in the human spleen (JPG, 114 KB) - (A) Binding of 1 µg/ml control Ig-EBNA1, αDEC-205 or αDEC-205-EBNA1 to CHO-Neo, CHO-huDEC-205, and immature monocyte-derived human DCs. (B) Staining of human spleen sections were carried out with (i) αDEC-205, (ii) Ig-EBNA1 fusion, or (iii) αDEC-205-EBNA1 fusion antibodies in combination with an anti-CD3 antibody (iv–vi). T and B cell areas of the spleen sections are indicated in the lower panels. Figure S3. Staining of spleen sections from mice with reconstituted human immune system components were carried out with the appropriate isotype control antibodies or αDEC-205 in combination with an αHLA class II antibody (JPG, 73.3 KB) - This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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