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Blood, Vol. 113, Issue 6, 1250-1256, February 5, 2009
TGF-β as a candidate bone marrow niche signal to induce hematopoietic stem cell hibernation
Blood Yamazaki et al.
113: 1250
Supplemental materials for: Yamazaki et al
Files in this Data Supplement:
- Figure S1. Treatment of HSCs with PP2, an inhibitor specific for Src-family kinases, inhibits lipid raft clustering (JPG, 54.3 KB)
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Freshly isolated CD34−KSL HSCs were incubated in the presence of PP2 or a negative control, DMSO, for 30 min and then were stimulated with SCF and TPO for another 30 min. The cells were stained with DAPI (blue), CTxB (green), and an anti-phospho-c-Src antibody (red).
- Figure S2. Freshly isolated CD34−KSL HSCs were sorted clonally into 96-well micro-titer plates and incubated in the presence of SCF (50 ng/ml), TPO (50 ng/ml), and TGF-β1 (10, 5, 2, 1, 0.5, ng/ml) (JPG, 15.4 KB)
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At day 7 of culture, the colonies consisting of more than 50 cells were counted under an inverted microscope.
- Figure S3. Freshly isolated CD34−KSL HSCs were sorted clonally into 96-well micro-titer plates and incubated in the presence of SCF, TPO, and TGF-β1, TGF-β2, or TGF-β3 (JPG, 61 KB)
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At the indicated time points, cell viability and cell numbers were assessed under an inverted microscope.
- Figure S4. Freshly isolated CD34−KSL HSCs were sorted clonally into 96-well micro-titer plates and incubated in the presence of SCF and TPO with or without TGF-β1, or in the presence of SCF and IL-11 with or without TGF-β1 (JPG, 18 KB)
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At day 7 of culture, the colonies consisting of more than 50 cells were counted under an inverted microscope.
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