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Blood, Vol. 112, Issue 13, 4999-5006, December 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef CD44-specific antibody treatment and CD44 deficiency exert distinct effects on leukocyte recruitment in experimental arthritis Blood Hutás et al. 112: 4999 Supplemental materials for: Hutas et al Files in this Data Supplement: Video 1. Leukocyte-endothelium interactions in the inflamed ankle synovial venule of a WT BALB/c mouse (AVI, 3.59 MB) - The majority of leukocytes adhere tightly to the endothelium; fewer cells exhibit rolling. Video acceleration: 10×. Video 2. Leukocyte-endothelium interactions in the inflamed synovial vessel of a CD44 KO mouse (AVI, 3.58 MB) - The majority of leukocytes roll slowly on the endothelium. Acceleration: 10×. Video 3. Rapid changes in leukocyte-endothelium interactions and leukocyte morphology in the inflamed synovium following i.v. injection of anti-CD44 mAb IM7 (200 µg) into a WT mouse (AVI, 4.53 MB) - The time of IM7 injection is indicated in the movie. Note: Rolling interactions rapidly disappear after IM7 administration. Adherent cells, which show homogeneously R6G staining initially, exhibit a “disheveled” appearance due to binding of bright particles to their surface following IM7 injection. Acceleration: 10×. Video 4. Leukocyte rolling/adhesion in a synovial blood vessel before injection of anti-Gr-1 mAb (0 min) (AVI, 2.49 MB) - Leukocytes roll smoothly in this venule before mAb administration. Acceleration: 10×. Video 5. The same vessel (as shown in Video 4) 5 min after anti-Gr-1 mAb injection (200 µg) (AVI, 2.56 MB) - Rolling cells have disappeared. The few adherent cells keep capturing bright particles from the circulation. Acceleration: 10×. Video 6. Leukocyte rolling/adhesion in a synovial blood vessel before injection of rmP-selectin (0 min) (AVI, 1.74 MB) - Leukocytes roll smoothly before injection of rmP-selectin. Acceleration: 10×. Video 7. The same vessel (as shown in Video 6) 5 min after rmP-selectin-Fc injection (30 µg) (AVI, 1.78 MB) - Rolling cells are not seen. Bright particles (platelets) accumulate on the surface of groups of adherent cells. Acceleration: 10×. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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