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Blood, Vol. 113, Issue 4, 827-836, January 22, 2009
CD49d provides access to "untouched" human Foxp3+ Treg free of contaminating effector cells
Blood Kleinewietfeld et al.
113: 827
Supplemental materials for: Kleinewietfeld et al
Files in this Data Supplement:
- Figure S1. Yield and purity in comparison to the commercially available CD25+/CD127dim/−kit (JPG, 71.7 KB)
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(A) Yield is shown for CD25+/CD127dim/−kit (Miltenyi Biotech) and the CD49d/CD127-depletion. Bars represent the average of three independent experiments. (B) A representative double-staining CD25 vs. Foxp3 is shown for cells obtained with the CD25+/CD127dim/−kit and by CD49d/CD127 depletion. (C) Direct comparison of yield and purity of corresponding preparations obtained from the same donor.
- Figure S2. CD45RA/CD45RO distribution in comparison to the commercially available CD25+/CD127dim/−kit (JPG, 101 KB)
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(A) Distribution in total CD4+ cells. Staining are shown for CD45RO, CD45 RA, and CD49d. Note that only a subset of CD45RA-cells stains bright fro CD49d. (B) Comparison of the frequency of CD45RA+ CD4+ cells in the cell preparations obtained with the CD25+/CD127dim/−kit (middle panel) and the CD49d/CD127 depletion method in reference to distribution obtained by FACS gating on CD127−CD25+ CD4+ cells (upper panel). Staining are shown for three different donors; FACS gating for CD4+ cells before Treg isolation is indicated in the lower panel of (A).
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