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Blood, Vol. 112, Issue 13, 4935-4939, December 15, 2008
UV-C irradiation disrupts platelet surface disulfide bonds and activates the platelet integrin  IIbβ3
Blood Verhaar et al.
112: 4935
Supplemental materials for: Verhaar et al
Files in this Data Supplement:
- Figure S1. Reduction in platelet count and aggregate formation after UV-C irradiation (JPG, 51.6 KB)
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(A) Platelets, stored for one day in 30% plasma, were irradiated with UV-C light (1500 J/m2) and subsequently the platelet count was determined immediately (+UV-C), or 60 min (t=60′) after storage in T25 culture flasks under continuous shaking. UV-C irradiation of platelets was also performed in the presence of 1 µg/mL tirofiban (black bars). Data represent the mean ± SEM of 5 experiments. Statistical difference with untreated platelets is denoted by an asterix (P< 0.001, paired Student’s t-test); (B) Microscopic analyses of samples taken 60 min after UV-C irradiation in the absence or presence of 1 µg/mL tirofiban.
- Figure S2. αIIbβ3 activation by UV-C irradiation also occurs in permeabilized platelets (JPG, 24.2 KB)
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UV-C irradiation induces αIIbβ3 activation (as measured by PAC-1 binding) in intact platelets (open bars) and digitonin-permeabilized platelets (closed bars), while these platelet ‘ghosts’ fail to respond to thrombin or PMA stimulation. Data represent the mean ± SEM of 4 experiments.
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