|
|
Blood, Vol. 113, Issue 12, 2646-2654, March 19, 2009
A novel in vivo siRNA delivery system specifically targeting dendritic cells and silencing CD40 genes for immunomodulation
Blood Zheng et al.
113: 2646
Supplemental materials for: Zheng et al
Files in this Data Supplement:
- Figure S1. Preparation of siILs (JPG, 31.6 KB)
-
(A) A schematic representation of siRNA-bearing immunoliposomes (siILs) showing siRNA incorporated within liposomes that are conjugated to a DC-specific mAb and are endowed with molecular stealth by PEG2000 strands. (B) Liposomal size distribution. Stealth liposomes encapsulating CD40 siRNA were prepared as described in Materials and Methods. The size of formed siRNA-liposomes showed a mean diameter of approximately 73 nm, as determined by dynamic light scattering.
- Figure S2. In vivo CD40 gene silencing in LN DCs after administration of CD40-siILs (JPG, 29.5 KB)
-
Mice were intravenously injected with 15 µg of naked Cy3-siRNA, isotype IgG-coupled Cy3-siILs, NLDC-145–coupled Cy3-siILs, or with PBS. 48 h after treatment, LN CD11c+ DCs were isolated using MACS beads. Cells were stained with Cy5PE-labed anti-CD40 mAb and the expression of CD40 were detected by flow cytometry. Data presented are representative of 4 independent experiments (n = 3 or 4 mice/group).
- Figure S3. Gene-specific silencing of CD40-siILs (JPG, 48.7 KB)
-
Mice were intravenously injected with 15 µg of naked Cy3-siRNA, isotype IgG-coupled Cy3-siILs, NLDC-145–coupled Cy3-siILs, or with PBS. 48 h after treatment, CD11c+ DCs were isolated from spleen (A) and lymph nodes (B) using MACS beads. Cells were stained with FITC-labeled anti-MHC II mAb. The expression of MHC II were detected by flow cytometry. Data presented are representative of 4 independent experiments (n = 3 or 4 mice/group).
|
|
