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Blood, Vol. 113, Issue 19, 4556-4565, May 7, 2009
Regulatory T cells differentially modulate the maturation and apoptosis of human CD8+ T-cell subsets
Blood Nikolova et al.
113: 4556
Supplemental materials for: Nikolova et al
Files in this Data Supplement:
- Figure S1. Phenotype of Treg cells (JPG, 62.2 KB)
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CD4 T-cell fractions, CD4+CD25hi (A) and CD4+CD25− (B), obtained as described in Materials and methods section, were studied for the intracellular expression of FoxP3. On the average, over 80% of CD4+CD25hi cells (C) and below 5% of CD4+CD25− (D) expressed FoxP3. A representative example is shown, the markers are set according to FoxP3 isotype control.
- Figure S2. Definition of CD8 T-cell subsets (JPG, 97.4 KB)
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PBMC were stained with one of the following mAb combinations: CD28/CD27/CD8/CD45RA; CCR7/CD27/CD8/CD45RA, CD160/ CD27/CD8/CD45RA. CD8 subsets were defined as follows: CD27+CD45RA+ (naïve); CD27highCD45RA− (central memory), CD27lowCD45RA+∕− memory subsets in transition to effectors, CD27−CD45RA− (effector memory) and CD27−CD45RA+ (terminal effectors), and expression of CD28 (A–F); CCR7 (G–L) and CD160 (M–R) was studied in each of these subsets. A representative example is shown.
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