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Blood, Vol. 113, Issue 9, 2003-2013, February 26, 2009
Modification of the cysteine residues in I B kinase and NF-B (p65) by xanthohumol leads to suppression of NF-B–regulated gene products and potentiation of apoptosis in leukemia cells
Blood Harikumar et al.
113: 2003
Supplemental materials for: Harikumar et al
Files in this Data Supplement:
- Figure S1. Xanthohumol does not inhibit TNF-induced AP-1 and SP-1 (JPG, 84.1 KB)
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(A) Xanthohumol does not inhibit TNF-induced AP-1 activation in vivo. KBM-5 (2 × 106 cells/well) were treated with different concentrations of xanthohumol for 4 h and then treated with 0.1nM TNF for 30 min, prepared NE and the activation of AP-1 was analyzed by EMSA (15 µg/well for EMSA). (B) Xanthohumol does not inhibit TNF-induced SP-1 activation in vivo. KBM-5 (2 × 106 cells/well) were treated with different concentrations of xanthohumol for 4h and then treated with 0.1nM TNF for 60 min, prepared NE and the activation of SP-1 was analyzed by EMSA. (C) Xanthohumol does not inhibit TNF-induced AP-1 activation in vitro. KBM-5 (2 × 106 cells/well) were treated with 0.1nM TNF for 30 min, prepared NE and incubated with different concentrations of xanthohumol for 30 min at 37°C, and the activation of AP-1 was analyzed by EMSA. (D) Xanthohumol does not inhibit TNF-induced SP-1 activation in vitro. KBM-5 (2 × 106 cells/well) were treated with 0.1nM TNF for 60 min, prepared NE and incubated with different concentrations of xanthohumol for 30 min 37°C, and the activation of SP-1 was analyzed by EMSA.
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