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Blood, Vol. 113, Issue 2, 389-395, January 8, 2009
Distinct roles of helper T-cell subsets in a systemic autoimmune disease
Blood Hoyer et al.
113: 389
Supplemental materials for: Hoyer et al
Files in this Data Supplement:
- Figure S1. Cytokine and transcription factor expression (JPG, 36.9 KB)
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Lymph node CD4+ T cells were purified and analyzed by RT-PCR. Data were normalized to HPRT, and WT was set to 1.
- Figure S2. Lymphoproliferation in IL-2-KO and IL-2/IFNγ-KO mice (JPG, 74 KB)
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(A) Bar graphs indicate the number (×10−6) of total and CD4+ lymphocytes at 3–4 weeks. (B) The percentages of CD4+ LN T cells expressing the activation marker CD62L and CD44 were determined by flow cytometry. *, p<0.0005; **; p<0.005; ***, p<0.05.
- Figure S3. Decreased percentage, but not total Treg numbers in IL-2-KO LN (JPG, 47.3 KB)
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Peripheral LN from 3–4-week-old and 3-month-old mice were stained for surface CD4 and intracellular Foxp3 expression. The percentage (A) and total number (B) of CD4+Foxp3+ cells are shown. There are no live IL-2-KO mice at 3 months, therefore no data for these mice are shown at the late time point. Bar graphs represent the means +/−SD of 5–6 mice from independent experiments.
- Figure 4. Th17 gene expression (JPG, 48.2 KB)
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CD4+ T cells from mesenteric lymph nodes were purified, treated with or without PMA + Ionomycin for 5 hours and analyzed by RT-PCR. Data were normalized to HPRT, and WT was set to 1.
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