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Blood, Vol. 113, Issue 2, 328-337, January 8, 2009
Staphylococcal SSL5 inhibits leukocyte activation by chemokines and anaphylatoxins
Blood Bestebroer et al.
113: 328
Supplemental materials for: Bestebroer et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 66.9 KB)
- Figure S1. Neuraminidase treatment does not affect binding of antibodies against chemokine receptors to cells (JPG, 84.6 KB)
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Binding of α-CXCR1, α-CXCR2, and α-C5aR mAbs to neutrophils, α-CXCR4 mAb to Jurkat cells, and α-CCR1 and α-CCR2 mAbs to monocytes was measured to untreated (black) or neuraminidase-treated (red) cells.
- Figure S2. U937 cells do not express chemokine receptors (JPG, 128 KB)
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U937 cells were stained with α- α-C5aR, α-CXCR1, α-CXCR2, α-CXCR4, α-CCR1, α-CCR2, and α-CCR5 mAbs, while U937-CXCR2 cells were stained with α-CXCR1 and α-CXCR2 mAbs. Histograms depict binding of these antibodies (black) with their isotype controls (red).
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