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Blood, Vol. 112, Issue 8, 3264-3273, October 15, 2008
The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement
Blood Caminschi et al.
112: 3264
Supplemental materials for: Caminschi et al
Files in this Data Supplement:
- Figure S1. Alignment of the Clec9A amino acid sequence with that of other C-type lectins (JPG, 280 KB)
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Human and mouse CLEC9A sequence was compared to other proteins in the databases (National Center for Biotechnology Information; http://www.ncbi.nlm.nih.gov). Proteins with highest homology to (A) human CLEC9A and (B) mouse Clec9A were aligned using Clustal W (DNASTAR Lasergene, MegAlign). Sequence identity is highlighted in grey. The boxed-in residues comprise the C-type lectin domain. Human CLEC9A shares 23%, 27.3%, 24.6%, and 27.8%, identity with hCLEC12A (NP_963917.2), hCLEC12B (NP_995324.1), hDECTIN-1 (NP_922938.1), hOLR1 (hLOX) (NP_002534.1), respectively. Mouse Clec9A shares 24.5%, 27.9%, 22.8%, 26.3%, and 19.7% identity with mClec1a (NP_780735.1), mClec1b (NP_064369.1), mClec12B (gene ID 71183), mDectin-1 (NP_064392.2), and mNKG2D (NP_149069.1), respectively.
- Figure S2. Expression of CLEC9A on human and macaque blood DCs (JPG, 48.1 KB)
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Human and macaque (Macaca nemestrina) PBMCs were isolated as described in Materials and Methods and surface immunofluorescence labeled with mAb against HLADR (L243-PercP), a cocktail of PE-conjugated mAb against “Lineage” markers including CD3 (BW264156; T cells), CD14 (Tuk4; monocytes), CD19 (6D5; B cells) and CD56 (AF12-7H3; NK cells) and CLEC9A (3A4-FITC). Blood DCs were gated as HLADR+Lineage− and analysed for their expression of CLEC9A.
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