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Blood, Vol. 113, Issue 1, 85-94, January 1, 2009
Immunotransplantation preferentially expands T-effector cells over T-regulatory cells and cures large lymphoma tumors
Blood Brody et al.
113: 85
Supplemental materials for: Brody et al
Files in this Data Supplement:
- Figure S1. Immunotransplant induces a tumor-specific, anti-A20 CD8 T-cell immune response (JPG, 108 KB)
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Immunotransplant donor mice received CpG/CTX vaccination and recipient mice received 900cGy TBI as previously. Donor splenocytes and bone marrow were transferred to recipients along with a simultaneous boost of 106 CpG-A20 cells, i.e. cultured with CpG-1826 at 3ug/ml for 72 hours, then irradiated (50Gy). On day 15, either untreated mice or immunotransplant mice were bled and IFNγ producing tumor-specific CD8 T-cells were assayed by flow cytometry, as described (n=3), with the addition of overnight co-culture with either A20 or CT26. (A) Flow cytometry data are shown for 3 mice each and gated on CD8(+) live lymphocytes and data shown are percentages of that population. (B) Mean values, standard deviations, and statistical significance per unpaired student t-test of data in A.
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