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Blood, Vol. 113, Issue 13, 3092-3101, March 26, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation Blood Radke et al. 113: 3092 Supplemental materials for: Radke et al Files in this Data Supplement: Table S1. Human blood eosinophils constitutively express Jagged 1 and differentially express Jagged 2, Delta 1, and Delta 4 (PDF, 32 KB) Figure S1. (A) Notch 1 extracellular domain was immunoprecipitated from whole eosinophil lysates (JPG, 42.6 KB) - Total Notch receptor 1 (B) and Jagged 1 (C) expression was compared by flow cytometry between eosinophils and CD4+ T cells isolated from peripheral blood of the same donor. (D) Freshly isolated cells from 2 of 6 donors examined expressed punctate, surface expression of J1. Figure S2. GSI dose-dependently promotes eosinophil viability (JGP, 41.6 KB) - Eosinophils cultured overnight in the presence of increasing doses of GSI were recovered and incubated on ice with PI for 30 min before analysis of PI incorporation by flow cytometry. Data are shown as % viable cells (PI−) ± SD. * p<0.05. Figure S3. GSI dose-dependently inhibits GM-CSF–induced eosinophil shape change (JPG, 69.9 KB) - (A) Data from Fig. 1A are displayed as FSC histograms. (B) Eosinophils were stimulated overnight in medium alone (NS) or with 100 pM GM-CSF in the presence of increasing doses of GSI before fixation and cell shape analysis. Data are expressed as average FSC geometric mean ± SD. * p<0.05. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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