SEARCH:   Advanced

Blood, Vol. 112, Issue 13, 4843-4852, December 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Stable transduction of quiescent T cells without induction of cycle progression by a novel lentiviral vector pseudotyped with measles virus glycoproteins Blood Frecha et al. 112: 4843 Supplemental materials for: Frecha et al Files in this Data Supplement: Table S1. Concentration rates of MV glycoprotein pseudotyped lentiviral vectors (PDF, 44.2 KB) Figure S1. Purified resting cord blood or adult CD3+ T cells were stimulated for 3 days with rIL-7 and subsequently transduced with HΔ24/FΔ30- or VSV-G–pseudotyped HIV-GFP vectors at an MOI of 10 (JPG, 56.9 KB) - Transduced cells were maintained in culture for 3 days in the presence of rIL-7. Cell cycle progression was monitored prior to and 3 days post-transduction by simultaneously visualizing the RNA (Pyronine-Y) and DNA (7-AAD) content of the T cells. The percentages of cells in the G0/G1a, G1b, S/G2/M phase of the cell cycle are indicated in the dot blots. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020