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Blood, Vol. 113, Issue 11, 2434-2441, March 12, 2009
The strength of inhibitory input during education quantitatively tunes the functional responsiveness of individual natural killer cells
Blood Brodin et al.
113: 2434
Supplemental materials for: Brodin et al
Files in this Data Supplement:
- Figure S1. Activating receptors used for stimulation assays are expressed similarly in all strains used in this study (JPG, 97.3 KB)
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Surface staining for NK1.1, NKp46, NKG2D, and Ly49D activating receptors. One representative experiment of 2, numbers indicate % of NK cells/GeoMFI.
- Figure S2. No phenotypic differences found between NK cells from the different mice used in this study (JPG, 193 KB)
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One representative experiment of 2 showing the staining pattern of 14 phenotypic markers on NK cells from MHC−∕−, Db, Dd, KbDb, and KbDbDd mice. Expressed as % of NK cells/GeoMFI.
- Figure S3. No differences in %CD107+, IFN-γ+ or GeoMFI IFN-γ in unstimulated or PMA+Ionomycin-stimulated NK cells from MHC−∕−, Db, KbDb, and KbDbDd mice (JPG, 76.2 KB)
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One representative experiment of 7 showing the staining of CD107 and IFN-γ in naïve NK cells from MHC−∕−, Db, KbDb, and KbDbDd mice either without any stimulation (PBS) or unspecific stimulation with PMA+Ionomycin.
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