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Blood, Vol. 113, Issue 7, 1513-1521, February 12, 2009
Targeting PKC: a novel role for beta-catenin in ER stress and apoptotic signaling
Blood Raab et al.
113: 1513
Supplemental materials for: Raab et al
Files in this Data Supplement:
- Table S1. Genes enza-regulated 2-fold (XLS, 93 KB)
- Figure S1. Accumulated b-catenin is transcriptionally active (JPG, 96.3 KB)
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(A) Inhibition of translation does not block enzastaurin-induced upregulation of β-catenin. MM.1S cells were treated with 5 µM Enzastaurin, 50 µM cycloheximide, or both for 24h, followed by immunoblot analysis with indicated antibodies. (B) MM.1S cells were exposed to increasing concentrations of enzastaurin (8h), and cell lysates were immunoprecipitated for b-catenin followed by immunoblotting with indicated antibodies. (C) Indicated cell lines were transiently transfected with the TOPFLASH reporter plasmid and treated with enzastaurin for 8h. Luciferin-induced luminescence is expressed as x-fold of control treated cells.
- Figure S2. Protein profiling of MM cell lines after treatment with enzastaurin (JPG, 69.3 KB)
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OPM2 and RPMI 8226 cells were exposed to enzastaurin at increasing concentrations, followed by immunoblotting with indicated antibodies.
- Figure S3. Ectopic overexpression of p73 induces cell death in MM cells (JPG, 33.6 KB)
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MM.1S cells were transiently transfected with transcriptionally active p73 (pTAp73) or empty vector, followed by either MTT assay 48h post transfection (top) or immunoblotting with indicated antibodies. Cells were transfected with 5∞g (+) or 10∞g (++) of plasmid-DNA. Control-transfected cells were additionally exposed to indicated concentrations of enzastaurin.
- Figure S4. Apoptosis associated with low-dose Enzastaurin, but not low-dose Bortezomib, induces upregulation of catenin and c-Jun (JPG, 41.6 KB)
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MM.1S cells were treated (16h) with either Enzastaurin (2.5 µM) or Bortezomib (5nM), followed by immunoblotting with indicated antibodies.
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