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Blood, Vol. 112, Issue 12, 4475-4484, December 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Biologic properties and enucleation of red blood cells from human embryonic stem cells Blood Lu et al. 112: 4475 Supplemental materials for: Lu et al Files in this Data Supplement: Document 1. Supplemental materials and methods (PDF, 84.1 KB) Figure S1. Expression of glyphorin A in hESC-derived erythroid cells (JPG, 73.4 KB) - Cytospin samples of hESC-derived erythroid cells collected from day 28 differentiation and maturation cultures were stained with human CD235a antibody. Almost 100% of cells stained positive for CD235a. (200×). Figure S2. Expression of β-globin chain in hESC-derived erythroid cells (JPG, 92.9 KB) - Cytospin samples of hESC-derived erythroid cells collected from day 28 differentiation and maturation cultures were stained with human β-globin chain specific antibody. (200×). Figure S3. Analysis of β-cluster globin gene expressions by RT-PCR (JPG, 34.1 KB) - Erythroid cells differentiated at different stages were collected and the expression of β-, γ- and ε-globin genes was analyzed by RT-PCR using globin chain specific primers. RNA from adult bone marrow cells was used as a positive control for β-globin gene and a negative control for ε-globin gene. Day28a and Day28b are erythroid cells from two separate experiments. BM, bone marrow. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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