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Blood, Vol. 112, Issue 12, 4512-4522, December 1, 2008
Endoglin expression in blood and endothelium is differentially regulated by modular assembly of the Ets/Gata hemangioblast code
Blood Pimanda et al.
112: 4512
Supplemental materials for: Pimanda et al
Files in this Data Supplement:
- Figure S5. Flowcytometry plots of E12. 5 Fetal livers from transgenic (TG+ve) and non- transgenic embryos from a P/LacZ/+15 transgenic line (L9203) × wild type cross (JPG, 27.0 KB)
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The P/LacZ/+15 transgene does not target FL hematopoietic cells.
- Figure S6. A representative E11.5 F0 transgenic embryo generated by pro-nuclear injection with P/LacZ/+18 (JPG, 13 KB)
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The number of embryos with endothelial and hematopoietic tissue staining relative to the total number of transgenic embryos is also shown.
- Figure S7. L1091 time course showing β galactosidase staining of E7.5-13.5 embryos (JPG, 75.5 KB)
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E11.5 staining is shown in Fig 3 of the main text.
- Figure S8. Flowcytometry of FDG treated non-transgenic (A) and transgenic (B) E12.5 FL cells from a litter of −8/P/lacZ/+7/+9 (L1091) × WT crosses (JPG, 50.5 KB)
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Cells that express the transgene (B) are enriched in CD150+ cells, which contain most of the long term repopulating HSCs in FL (Kim, I., 2006. Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors. Blood 108(2): 737-44).
- Figure S9. −8/P/lacZ/+7/+9 transgene expression in adult mice (L1091) (JPG, 58.8 KB)
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The transgene is not expressed in the bone marrow of adult mice aged 8 months.
- Figure S10. Quantitative PCR for Endoglin, Fli1, Gata2, and Pu.1 expression plotted relative to βactin expression in HPC-7 (JPG, 41.7 KB)
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