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Blood, Vol. 113, Issue 2, 412-421, January 8, 2009
Transcriptional repression of microRNA genes by PML-RARA increases expression of key cancer proteins in acute promyelocytic leukemia
Blood Saumet et al.
113: 412
Supplemental materials for: Saumet et al
Files in this Data Supplement:
- Table S1. Set of primers and LNAs used in this study (PDF, 36.6 KB)
- Table S2. cDNA microarray results: up-regulated mRNAs upon ATRA (1 µM, 16 hours) in APL cell lines (XLS, 55.5 KB)
- Table S3. cDNA microarray results: down-regulated mRNAs upon ATRA (1 µM, 16 hours) in APL cell lines (XLS, 54 KB)
- Figure S1. NB4-LR1 and NB4-LR2 cells (JPG, 61.1 KB)
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(A) Morphology of MGG-stained of NB4-LR1 and NB4-LR2 cells treated or not with ATRA (1µM) for 4 days. Cell maturation was monitored by NBT-dye reduction assay and the percentage of NBT positive cells at 4 days of treatment is indicated (bottom left). (B) NB4-LR1 and NB4-LR2 cells were treated with ATRA (1 µM) for 16 hours. RNAs used in Figure 6A were also analyzed by RT-qPCR directed against RARB mRNA. Results are indicated as fold change, 1 being the value obtained in absence of treatment.
- Figure S2. RT-qPCR directed against the HOXB8 mRNA (JPG, 45.1 KB)
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(A) RT-qPCR directed against the HOXB8 mRNA performed on RNAs extracted from NB4, NB4-LR1, and NB4-LR2 treated or not with 1mM ATRA for 16 hours. (B) RT-qPCR directed against the HOXB8 mRNA performed on RNAs extracted from 293T transfected with LNAs targeting the miR-32 or the miR-377. The results are expressed as fold change, 1 being the value obtained with the control LNA.
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