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Blood, Vol. 113, Issue 5, 1097-1104, January 29, 2009
STIM1 is essential for Fc receptor activation and autoimmune inflammation
Blood Braun et al.
113: 1097
Supplemental materials for: Braun et al
Files in this Data Supplement:
- Figure S1 (JPG, 69.6 KB)
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(A) Staining of wild-type or FcγRI−∕− PM with anti-FcγRI-Alexa 647 (clone 290322). (B) Staining of wild-type or FcγRIII−∕− PM with anti–FcγRIII-Alexa 647 (clone 275003).
- Figure S2. Reduced FcγRIII levels but unaltered phagocytosis in FcRγ+/− macrophages (JPG, 49.2 KB)
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(A) Staining of wild-type or FcRγ+/− PM with anti–FcγRIII-Alexa 647 (clone 275003). Results are MFI ± S.D. (n=4 per group). (B, C) PM cells were incubated with uncoated (control) or IgG2a¬coated MRBC. (B) To study binding, incubations were performed for 4 hrs at 4°C. To study phagocytosis (C), incubations were performed for 4 hrs at 37°C followed by the lysis of extracellular MRBC. The percentage of positive cells that formed rosettes with more than three erythrocytes or ingested more than one erythrocyte was assessed microscopically. The results shown are mean ± SEM of 2 independent experiments performed in duplicate. No significant differences were in binding or phagocytosis were noted between wild-type and FcRγ+/− PM.
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