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Blood, Vol. 113, Issue 3, 585-593, January 15, 2009
Hepatitis C virus association with peripheral blood B lymphocytes potentiates viral infection of liver-derived hepatoma cells
Blood Stamataki et al.
113: 585
Supplemental materials for: Stamataki et al
Files in this Data Supplement:
- Figure S1. Effect(s) of stimulation on B-cell association with JFH-1 (JPG, 55.6 KB)
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Untreated and CD40L/IL-4 stimulated L3055-Bcl-2 and KEM cells were incubated with JFH-1 for 2h at 37°C, unbound virus removed by washing and cells analyzed for HCV RNA by RT-PCR (A) or trans-infection of Huh-7.5 (B). Error bars represent the SD from 3 or 4 replicate infections.
- Figure S2. Comparative infectivity of cell-free and B-cell–associated JFH-1 (JPG, 26.8 KB)
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Infectivity of cell-free (open circle and dotted line) and CD40L/IL-4–stimulated L3055-Bcl-2 (filled circle) and PBMC derived B-cell–associated JFH-1 (BD1-open, BD2-filled squares) at 37°C for different time periods. Infectivity is expressed relative to cells co-cultured with Huh-7.5 immediately following virus loading. The relative infectivity retained by primary B cells following incubation at 37°C for 2.5 and 4.5 hours is significantly higher than that retained either by cell-free virus or L3055-Bcl-2 cells (Dunn’s test, p=<0.05).
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